Table 1.

Bacterial strains used in this study

StrainaDescriptionReference and source
PA103Wild-type, cytotoxic isolate 22
PA103exsA::ΩMutant of PA103 defective in expression of type III secretion genes 11
PA103pscJ::Tn5 Mutant of PA103 defective in production of PscJ, a putative component of the bacterial type III secretion apparatus 15, 20
PA103pcrG::Tn5 Mutant of PA103 defective in secretion of PopB and PopD, putative components of the type III translocation complex 15, 20
PA103exoU::Tn5 Mutant of PA103 defective iin production of ExoU 14, 20
PA103exoU::Tn5(pAH807)PA103exoU::Tn5 harboring a plasmid containing an intact copy of the exoU gene and its chaperone, SpcU 6, 14, 20
PA103tox::ΩMutant of PA103 defective in production of exotoxin A 21
PAK P. aeruginosa laboratory strain 37
6294 P. aeruginosa hyperinvasive corneal isolate 9
PAO1 P. aeruginosalaboratory strain 17
388 P. aeruginosa laboratory strain 2, 18
617000 P. aeruginosa respiratory isolate 14
M90TWild-type S. flexneri serotype 5 41; a generous gift from Arturo Zychlinsky
BS176Plasmid-cured derivative of M90T 41; a generous gift from Arturo Zychlinsky
  • a Tn5 refers to a Tn5 transposon modifed to contain the gentamicin resistance gene (20).