TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesReference or source
Yersinia pestis strains
    201Wild-type strain48
    201 ΔyopKyopK gene of strain 201 replaced by a kanamycin cassette using the λRed recombinant systemThis study
    201 ΔyopK-CyopKpACYC-yopK electroporated into 201 ΔyopKThis study
    201 ΔyopK-CyopKΔ91–124pACYC-yopKΔ91–124 electroporated into 201 ΔyopKThis study
    201/YopE-TEMpBBR1-YopE-TEM electroporated into 201This study
    201/YopK-TEMpBBR1-YopK-TEM electroporated into 201This study
    201/TEMpBBR1-TEM electroporated into 201This study
    201 ΔyopK/YopE-TEMpBBR1-YopE-TEM electroporated into 201 ΔyopKThis study
    201 ΔyopK-CyopK/YopE-TEMpBBR1-YopE-TEM electroporated into 201 ΔyopK-CyopKThis study
    ΔyopK-CyopKΔ91–124/YopE-TEMpBBR1-YopE-TEM electroporated into 201 ΔyopK-CyopKΔ91–124This study
Plasmids
    pET-YopK-GFPyopK gene fused with GFP-coding sequence and cloned into pET28aThis study
    pET-YopKΔ91–124-GFPyopK gene lacking coding sequence of amino acids 91–124 fused to GFP gene and cloned into pET-28aThis study
    pGEX-YopKyopK gene cloned into pGEX-4T-2This study
    pGEX-YopK1–60Coding sequence of amino acids 1–60 of YopK cloned into pGEX-4T-2This study
    pGEX-YopK1–124Coding sequence of amino acids 1–124 of YopK cloned into pGEX-4T-2This study
    pGEX-YopK61–124Coding sequence of amino acids 61–124 of YopK cloned into pGEX-4T-2This study
    pGEX-YopK91–182Coding sequence of amino acids 91–124 of YopK cloned into pGEX-4T-2This study
    pGEX-YopKΔ91–124yopK gene lacking coding sequence of amino acids 91–124 cloned into pGEX-4T-2This study
    pACYC-yopKCoding sequence of yopK with ∼300 bp of upstream sequence cloned into pACYC184This study
    pACYC-yopKΔ91–124Coding sequence of yopK lacking bp 271–372 and ∼300 bp of upstream sequence cloned into to pACYC184This study
    pBBR1MCSEmpty vector for construction of TEM-1 beta-lactamase reporterLab collection
    pBBR1-TEMTEM-1 beta-lactamase-coding sequence cloned into pBBR1MCS vectorThis study
    pBBR1-YopE-TEMCoding sequence and ∼300 bp upstream sequence of yopE cloned into pBBR1-TEM directly upstream of TEM-1-coding sequenceThis study
    pBBR1-YopK-TEMCoding sequence and ∼300 bp upstream sequence of yopK cloned into pBBR1-TEM directly upstream of TEM-1-coding sequenceThis study