Table 1.

Optimization of culture conditions for toxin production by V. cholerae WO7

MediumCulture conditionsDI
30°C37°C
BHIStationary, 24 h0.40 ± 0.080.59 ± 0.10
Stationary, 4 h; with shaking, 16 to 18 h0.44 ± 0.060.64 ± 0.02
TSBStationary, 24 h0.9 ± 0.041.20 ± 0.08
Stationary, 4 h; with shaking, 16 to 18 h1.10 ± 0.091.34 ± 0.05
CAYEStationary, 24 h0.33 ± 0.110.56 ± 0.07
Stationary, 4 h; with shaking, 16 to 18 h0.42 ± 0.040.42 ± 0.12
AKIStationary, 24 h1.15 ± 0.151.50 ± 0.18
Stationary, 4 h; with shaking, 16 to 18 h1.20 ± 0.141.60 ± 0.22