Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
E. coli
 DH5αHost strain for cloning experiments 54
 HB101Host strain essential for propagating plasmids carrying mutated H. ducreyi DNA inserts used in electroporation 6, 54
 XL1-BlueHost strain used for screening of the ZAP Express bacteriophage libraryStratagene
 XLOLRHost strain used for excision of recombinant pBK-CMV plasmids from the ZAP Express bacteriophageStratagene
H. influenzae
 NTHI 6564Wild-type parent strain 39
 NTHI 6564 pzp1 mutant pzp1 mutant of NTHI 6564 39
 NTHI 6564.pzp1(pCW170)NTHI 6564 pzp1 mutant containing the vector pCW170This study
 NTHI 6564.pzp1(pDL6-2)NTHI 6564 pzp1 mutant containing pDL6-2 with the H. ducreyi znuA geneThis study
H. ducreyi
 35000Wild-type strain isolated in Winnipeg, Manitoba, Canada 29
 35000.901Isogenic mutant with a kan2 cartridge inserted into the MscI site in the znuAgeneThis study
 35000.901(pCW170)35000.901 containing the vector pCW170This study
 35000.901(pDL6-2)35000.901 containing pDL6-2 with the H. ducreyi znuA geneThis study
 RO18Wild-type strain from Kenya 3
 Cha-1Wild-type strain from Dallas, Tex. 31
 A77Type strain from Institut Pasteur 44
 1151Wild-type strain from the Gambia 57
 1153Wild-type strain from the GambiaAllan Ronald
 511Wild-type strain from ThailandAllan Ronald
 512Wild-type strain from Thailand 57
 Hd12Wild-type strain from Korea 43
 1352Wild-type strain from Kenya 57
 78226Wild-type strain from Winnipeg, Manitoba, Canada 3
 6VWild-type strain from Atlanta, Ga. 3
 BG411Wild-type strain from Kenya 3
 O41Wild-type strain from Sweden 57
 1145Wild-type strain from Amsterdam 57
Plasmids
 pUC18Cloning vector, Ampr 54
 pUC18K2pUC18 with the kan2 cartridge containing the promoterlessaphA-3 geneJames Kaper
 pBK-CMVPhagemid excised from the ZAP Express cloning vectorStratagene
 pJKT913pBK-CMV with a 3.7-kb H. ducreyi DNA insert containing the znuA geneThis study
 pBluescript II KS(−)Cloning vector, AmpStratagene
 pJKT933pBluescript II with a 3.7-kbHindIII-SacI fragment from pJKT913 containing the znuA geneThis study
 pDL5-3pJKT933 with akan2 cartridge inserted into the MscI site within the H. ducreyi znuA ORFThis study
 pLS88Cloning vector capable of replication in H. ducreyi; Kanr SmrSulr 19
 pCW170pLS88 with acat cartridge inserted into the EcoRI site of this vectorThis study
 pDL6-2pCW170 with a 402 ntPvuI-XhoI fragment removed from thekan gene and a 1.8-kb PCR-derived,XhoI-PvuI DNA fragment with the H. ducreyi znuA gene inserted in its placeThis study