Table 1.

Recognition of mycobacterial antigens by mouse memory effector cellsa

ExptAntigenbMean IFN-γ releasec (ng/ml) ± SE
IST-CF21.4 ± 0.9
Ag85B6.5 ± 0.2
nGroES (I)6.5 ± 0.2
rGroES (Mt 10-2)0.4 ± 0.1
rGroES (Mt 10-1B)0.3 ± 0.1
rGroES (Ml 10-2)0.3 ± 0.1
rGroES (Ml 10-1B)0.4 ± 0.2
IIST-CF22.9 ± 2.7
nGroES (II)3.2 ± 0.3
rGroES (Mt 10-2)0.4 ± 0.1
rGroES (Mtcpn10)0.5 ± 0.0
sGroES0.4 ± 0.0
  • a Splenic lymphocytes were isolated 4 days after reinfection with 106 M. tuberculosisand stimulated in vitro.

  • b All antigens were used at a concentration of 5 μg/ml. nGroES (I) was purified by preparative electrophoresis; nGroES (II) was purified by column chromatography; rGroES (M. tuberculosis) was expressed in E. coli either in the pMAL system (Mt 10-2 and Mt 10-1B) or in the T7 expression system (Mtcpn10); rGroES (M. leprae) was expressed in the pMAL system (Ml 10-2 and Ml 10-1B); sGroES was the synthetic version ofM. tuberculosis GroES.

  • c Stimulation without antigen gave IFN-γ values of <0.05 ng/ml.