Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference
E. coli
 042Wild-type EAEC strain from Peru 50
 HB101K-12/B hybrid 7
 KS474FΔlacX74 galE galK thi rpsL (strA) ΔphoA (PvuII) degP41PstI-Kanr) 4
 UT5600F ara-14 leuB6 azi-6 lacY1 tsx67 proC leu-6 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1 entA403Δ(ompT-fecC266) ΔompP 41
 JCB517 araD139 Δ(araABC-leu)7679 galU galK Δ(lac)X74 rpsL thi phoR zih12::Tn10 dsbA::Kan-1 81
S. flexneri
 2457TWild-type S. flexneri 2a 21
Plasmids
 pACYC184Small low-copy-number cloning vector (Camr, Tetr) 12
 pPic15.8-kb EcoRI-EcoRV chromosomal fragment of EAEC 042 cloned into pACYC184 (Tetr)This study
 pPicS258IpPic1 (Tetr) S258 to I258 This study