Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaReference
Pseudomonas aeruginosa
 PAO1Wild-type, prototrophic strain; clinical isolate16
 PAO-R1PAO1 ΔlasR Tcr12
 PDO100PAO1 ΔrhlI::Tn501Hgr5
 JP1PAO1 ΔlasI Tcr30
 JP2PDO100 ΔlasI HgrTcr30
Escherichia coli DH5αsupE44 thi-1 recA1 gyrANalr1
Plasmids
 pSW200pUC18 cloning vector carrying 1.8-kb PstI fragment that allows plasmid ColE1 to replicate stably in P. aeruginosa12
 pLASI-2Cbr; pSW200 carrying 989-bp fragment which contains lacP-lasI26
 pJPP42pLASI-2 carrying 1.5-kb fragment which containslacP-rhlI30
 pJPP45pSW200 carrying 1.5-kb fragment which containslacP-rhlI30
  • a Abbreviations: Tcr, tetracycline resistance; Hgr, mercuric chloride resistance; Nalr, nalidixic acid resistance; Cbr, carbenicillin resistance.