Table 4.

Stability within CVD 908-htrA ofori15A replicons, containing plasmid maintenance systems of increasing complexity, grown without selection and in the presence of increasing osmolaritya

Strain or plasmidStability result with NaCl concn:
50 mM150 mM300 mM
OD600b% Fluorescing bacteriaMean fluorescence intensitycOD600% Fluorescing bacteriaMean fluorescence intensitycOD600% Fluorescing bacteriaMean fluorescence intensityc
Strain
 CVD 908-htrA 0.981000.61.111000.61.121000.6
Plasmids
 pGEN911.0013.228.61.1711.442.91.2610.965.5
 pGEN1111.2647.451.81.1728.993.61.1242.465.1
 pGEN1211.0180.553.31.2073.874.01.1556.7105.3
 pGEN1931.1171.450.91.2465.264.71.2253.790.8
 pGEN2221.0196.852.11.2893.367.81.1395.389.2
  • a These data are represented by histograms in Fig. 2.

  • b OD600, optical density at 600 nm.

  • c All standard error values are <0.6.