Table 2.

Phagocytosis and oxidative burst responses mediated by anti-TbpA+B serum opsonins in meningococcal disease patients

Parameter and patient groupaMedian (range) for beads coated with TbpA+B from strain:
K454B16B6
AdmissionIntermediateb6 wkAdmissionIntermediateb6 wk
% Phagocytosing nonlymphocytesc
 I6 (4–7)37 (24–57)19 (11–30)41 (7–94)53 (16–97)55 (12–97)
 II6 (4–20)33 (6–65)18 (9–37)45 (4–87)52 (8–91)53 (11–90)
 III6 (3–15)34 (18–65)22 (17–32)29 (9–98)42 (30–98)50 (14–98)
 IV5 (4–13)47 (24–75)25 (9–45)41 (7–87)95 (39–97)92 (39–98)
 V6 (4–12)58 (8–86)29 (6–56)25 (6–67)57 (6–93)35 (4–72)
Beads/phagocytosing nonlymphocyte (mean no.)
 I1.3 (1.1–1.7)1.9 (1.4–2.3)1.4 (1.2–1.6)1.4 (1.2–5.6)3.9 (1.6–8.7)2.6 (1.3–7.1)
 II1.4 (1.3–1.6)1.8 (1.3–2.5)1.4 (1.2–1.7)1.5 (1.0–3.0)2.1 (1.3–6.8)1.3 (1.3–3.4)
 III1.4 (1.2–2.1)1.8 (1.5–2.7)1.4 (1.3–1.7)1.3 (1.2–10.3)2.6 (2.2–11.1)2.1 (1.5–9.1)
 IV1.3 (1.1–1.8)2.5 (1.6–3.5)1.5 (1.2–2.3)1.4 (1.1–3.0)11.9 (3.0–17.6)8.4 (2.7–16.6)
 V1.4 (1.0–1.6)2.1 (1.3–3.8)1.5 (1.3–2.2)1.2 (1.1–2.1)2.9 (1.1–8.5)1.8 (1.2–3.5)
PPd
 I7 (7–12)63 (37–128)27 (13–42)54 (8–522)199 (27–843)144 (16–690)
 II9 (6–26)57 (8–165)22 (14–52)69 (5–260)103 (10–614)71 (14–304)
 III8 (6–22)58 (27–177)31 (27–48)35 (11–1,015)108 (64–1,094)76 (20–890)
 IV6 (4–23)120 (36–262)37 (11–100)67 (8–265)1,130 (117–1,709)762 (103–1,623)
 V8 (4–18)123 (13–437)51 (7–121)29 (7–141)112 (7–785)62 (5–249)
Oxidative burste
 I0.2 (0.2–0.2)0.5 (0.3–1.1)0.3 (0.2–0.4)0.4 (0.2–2.1)1.6 (0.3–10.5)0.9 (0.2–7.4)
 II0.2 (0.2–3.3)0.5 (0.2–0.7)0.2 (0.2–0.5)0.5 (0.2–5.6)0.9 (0.2–3.9)0.4 (0.2–1.4)
 III0.2 (0.2–0.3)0.4 (0.2–0.7)0.3 (0.2–0.4)0.3 (0.3–13.0)0.7 (0.3–17.1)0.5 (0.2–13.7)
 IV0.2 (0.2–0.3)0.7 (0.3–1.0)0.3 (0.2–0.7)0.4 (0.2–0.9)11.5 (0.7–24.3)7.4 (0.6–24.7)
 V0.2 (0.2–0.3)0.5 (0.2–4.4)0.3 (0.2–0.8)0.2 (0.2–0.9)0.7 (0.2–10.1)0.4 (0.2–2.2)
  • a Allocation of patients into groups depending on serogroups, serotypes, and subtypes of meningococcal strains (Table 1).

  • b Serum samples from 39 patients on days 3 to 24 (median, day 15) (Table 1).

  • c Percent nonlymphocytes with associated bead fluorescence.

  • d Percent phagocytosing nonlymphocytes multiplied by mean number of beads per phagocytosing cell.

  • e Mean nonlymphocyte R-123 fluorescence.