Table 3.

CEME-related peptides inhibit LTA-stimulated production of TNF and IL-6 by RAW 264.7 cellsa

Peptide% Inhibition of cytokine induction (± SE) by:
E. coli LPS (100 ng/ml)bS. aureusLTAB. subtilis LTA (1 μg/ml)S. pyogenes LTA (1 μg/ml)
100 ng/ml1 μg/ml
TNFIL-6TNFIL-6TNFIL-6TNFIL-6TNFIL-6
PB989899 ± 198 ± 196 ± 193 ± 493 ± 291 ± 197 ± 199 ± 1
CP26909048 ± 1976 ± 152 ± 1250 ± 1523 ± 1742 ± 1168 ± 378 ± 6
CEME947699 ± 192 ± 286 ± 1092 ± 690 ± 294 ± 296 ± 198 ± 1
CEMA908294 ± 497 ± 291 ± 487 ± 286 ± 288 ± 196 ± 197 ± 2
CP29989699 ± 195 ± 390 ± 295 ± 376 ± 1489 ± 496 ± 199 ± 1
CM5424465 ± 288 ± 164 ± 1273 ± 877 ± 167 ± 380 ± 475 ± 5
CM7999599 ± 195 ± 196 ± 295 ± 483 ± 491 ± 690 ± 494 ± 5
CP203989098 ± 199 ± 190 ± 293 ± 370 ± 1477 ± 1287 ± 693 ± 3
CP207979392 ± 696 ± 183 ± 384 ± 1072 ± 1573 ± 1269 ± 1189 ± 3
CP2080746 ± 561 ± 114 ± 819 ± 926 ± 829 ± 523 ± 937 ± 13
CPα2939497 ± 189 ± 689 ± 585 ± 383 ± 588 ± 693 ± 195 ± 2
  • a RAW 264.7 cells were cultured with the indicated concentrations of LTA from different bacteria in the presence or absence of the various CEME-related peptides (20 μg/ml) or polymyxin B (PB; 20 μg/ml). After 6 h, cell supernatants were collected and analyzed for TNF-α and IL-6 content by ELISA. With the exception of those for E. coli, the data are presented as mean percent inhibition of cytokine production ± the standard error of the mean for triplicate samples. The 100% value ranged from 16 to 20 ng/ml for LPS and from 9 to 12 ng/ml for LTA. Values for medium-only controls as well as medium plus peptide were always less than 0.3 ng/ml.

  • b Data taken from reference (22).