Table 1.

Oligonucleotide primer sequences and their applications

DesignationSequenceApplicationTemplateAnnealing temp (°C)
ISS1fora5′-GGAGAGAATGGGTTCTGTTGCISS1probepGh9::ISS160
ISS1reva5′-GCTCTAGAGCATTCTCTGGTTC
P0505′-GTTATCGTTCACCGTTCCCS. pyogenes hasA probeS. pyogenes genomic DNA47
P0525′-TACGTGTTCCCCATTCCG
P064a5′-AGAACCGAAGAATTCGAACGCTCAmplification of S. uberis DNA and sequencing primersWild-type and mutant S. uberis 0140J genomic DNAs50
P067b5′-GGTGGAGCGTGCTGCTCA
P0705′-CAAAACGAATAGATGTATCAATCC
P082a5′-CCAACAGCGACAATAATCACATC
P084c5′-CAGTCCCGACGGTGTAAAACG
P085c5′-GCTAAAGAGGTCCCTAGACTCT
  • a Primers complementary to ISS1.

  • b A primer complementary to S. pyogenes hasA.

  • c Primers complementary to the pGh9 vector.