Table 1.

Colony hybridization studiesa

Strain CategorySerotype DECbHybridizationc with probe:Source or reference
ABCDEFG
HB101K-12+++Lab stock
DH5α K-12++Lab stock
MG1655K-12++ 6
E2348/69EPEC1 O127:H6 1+++++++ 33
JPN15 EPEC1 O127:H61+++++++ 25
#19 EPEC1O142:H6 +++++++ 44
#15EPEC1 O142:H6 +++++++ 44
572-56 EPEC1 O55:H61+w+w+++ 49
C54-58 EPEC1O55:H6 1++++++ 49
5513-56EPEC1O55:NM2+++++++ 49
3299-85EHEC1O157:H73+++ 49
3104-88EHEC1O157:H73+++ 49
C999-87EHEC1O157:H74+++ 49
5338-66EPEC20111:H216+++ 49
C142-54EPECO111:H126+++ 49
2277-67EPECO111:H126+++ 49
184-83EPEC2O111:NM6++++ 49
2198-77EPEC2O111:NM8+++ 49
C194-65EHEC2O111:H88+++ 49
3233-61EHEC2O26:H119+++ 49
2262-79EHECO26:HN9w+ 49
C814-67EHEC2O26:H119+++ 49
2254-75EPEC2O128:H211w+ 49
3733-71EPEC2O128:H211+ 49
A9619-c2EPEC2O45:H211++ 49
WM-63EPEC2O128:H211+ww++ 49
F1-50EPEC2O111:H212+++ 49
2966-56 EPEC2 O111:H212++++ 49
3350-73 ETECO128:H713+++ 49
5024-71 ETECO128:H713+++ 49
C500-74 ETEC O128:H713+++ 49
C916-70ETEC O128:H2114+++ 49
C691-71ETEC O128:H2114++ 49
#6 EPECO119:H6 ++++ 16
#15EPEC O119:H6 +++ 16
RDEC1 RDEC1+++Lab stock
B171EPEC O111:NM ++++Lab stock
BG6119 EHEC2 O26:H11+++Lab stock
EDL933EHEC1 O157:H7+++Lab stock
86-24EHEC1 O157:H7+++Lab stock
DK38EPEC O55:H7 +++Lab stock
DK154 EHEC O103+++Lab stock
Citrobacter rodentium 4280+Lab stock
  • a All strains tested were E. coli except for Citrobacter rodentium. All strains except K-12 and enterotoxigenic E. coli (ETEC) strains possessed the eae gene and presumably the LEE pathogenicity island. Hybridizations were performed at 65°C under standard conditions and repeated under lower stringency conditions, 50°C, with identical results.

  • b Diarrheagenic E. coli clones according to Whittam et al. 49.

  • c w, weak hybridization.