Table 1.

Antigen responsiveness of PBMC from uninfected andM. bovis-infected animals stimulated with control orM. bovis antigens as measured by lymphocyte proliferation and IFN-γ production from 21 to 63 days p.i.a

AnimalLymphocyte proliferation (cpm) in response to:IFN-γ production (OD450) in response to:
PBSMBSECFPBSMBSECF
Infected
 I1435 ± 11269,588 ± 8,80578,440 ± 9,4020.09 ± 0.021.87 ± 0.142.06 ± 0.24
 I2398 ± 12064,854 ± 9,99746,414 ± 9,8540.10 ± 0.012.36 ± 0.202.49 ± 0.19
 I3516 ± 18645,452 ± 3,53129,599 ± 9,5790.10 ± 0.013.01 ± 0.213.16 ± 0.26
Control
 U1–U3395 ± 92646 ± 445703 ± 3690.10 ± 0.010.13 ± 0.050.116 ± 0.02
  • a Experimental animals before M. bovis infection and control animals before the experiment displayed mean responses of <700 cpm and <0.10 OD450units in response to PBS, MBSE, CF, and avian and bovine PPD for lymphocyte proliferation and IFN-γ production, respectively. Results for infected cattle are means ± standard errors of the mean (SEM) of four repeated experiments for each animal. Results for control (uninfected) cattle are means ± SEM of the responses for three animals from four repeated experiments.