Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
E. coli
  SM10Mobilizing strain; transfer genes of RP4 integrated in chromosome; KmrSms 48
  SM10λpir SM10 with a λ prophage carrying the gene encoding the π protein 37
  SUREe14 (mcrA) Δ(mcrCB-hsdSMR-mrr)171 endA1 supE44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC[F′ proAB lacI q ZΔM15 Tn10] Kanr Tetr Stratagene
  DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 Bethesda Research Laboratories
  TOP10F mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr)endA1 nupG Invitrogen
  XL10-GoldTetrΔ (mcrA)183Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte[F′ proAB lacI q Z ΔM15 Tn10 (Tetr) Amy Camr]Stratagene
B. pseudomallei
  1026bClinical isolate; Kmr Gmr Smr PmrTcs Tps 16
  SR10151026b(pSR1015); SmrTcr This study
  SR10161026b(pSR1016); Smr Tcr This study
  DD5031026b derivative; allelic exchange strain; Δ(amrR-oprA)(Kms GmsSms) rpsL(Smr) 39
  PB401DD503 derivative; ΔfliC Brett et al., unpublished
  SR1001DD503 derivative; ΔfliC wbiE::aacC1 Gmr This study
  SLR5SR1001 derivative;wcbB::Tn5-OT182 Tcr This study
  SLR8SR1001 derivative;wzt2::Tn5-OT182 Tcr This study
  SLR13SR1001 derivative;wcbP::Tn5-OT182 Tcr This study
  SLR18SR1001 derivative;wcbE::Tn5-OT182 Tcr This study
  SLR19SR1001 derivative;wcbH::Tn5-OT182 Tcr This study
  SR201::TpDD503 derivative;wcbC::TpThis study
  SR202::TpDD503 derivative;wcbA::TpThis study
  SR203::TpDD503 derivative;yafJ::TpThis study
B. thailandensis E264Soil isolate; LPS contains only type II O-PS 7
B. mallei NCTC 10260
B. cepacia complex
B. cepacia CEP509 (genomovar I)CFaisolate, Australia 33
B. multivorans C5393 (formerly B. cepacia genomovar II)CF isolate, Vancouver, Canada 33
B. cepacia K56-2 (genomovar III)CF isolate, Toronto, Canada 15
B. stabilis (formerly B. cepacia  genomovar IV)
  CEP0717CF isolate, Calgary, CanadaH. Rabin
  CEP0467CF isolate, Edmonton, CanadaE. Mahenthiralingam
  J687Non-CF isolate, France 56
  CEP0726CF isolate, Calgary, CanadaH. Rabin
  LMG14291CF isolate, Belgium 56
  LMG7000Blood isolate, Sweden 56
  LMG14294CF isolate, Belgium 33
B. vietnamiensis (also known as B. cepacia genomovar V) LMG10929Rice root isolate, Vietnam 33
Plasmids
 pSKM11Positive selection cloning vector; IncP mob; ColE1 ori; AprTcs 38
 pPCRpBluescript II SK(+) derivative; Apr 3
 pZErO-2.1Positive selection cloning vector; ColE1; Kmr Invitrogen
 pPCR2.1-TOPOTopoisomerase-mediated cloning vector; Apr Kmr Invitrogen
 pDD1015Subtractive hybridization product cloned into pZErO-2.1; Kmr This study
 pDD1016Subtractive hybridization product cloned into pZErO-2.1; Kmr This study
 pSR1015 KpnI-XhoI fragment from pDD1015 cloned into pSKM11 Apr Tcr This study
 pSR1016 KpnI-XhoI fragment from pDD1016 cloned into pSKM11; Apr Tcr This study
 pSR1015Bg8-kb BglII fragment from SR1015 obtained by self-cloning; Apr Tcr This study
 pOT182pSUP102(Gm)::Tn5-OT182; Cmr Gmr AprTcr 36
 pSLR5B8-kbBamHI fragment from SLR5 obtained by self-cloning; Apr This study
 pSLR5H10-kbHindIII fragment from SLR5 obtained by self-cloning; Apr This study
 pSLR13H9-kbHindIII fragment from SLR13 obtained by self-cloning; Apr This study
 p34E-oriTpVector containing self-cloning Tp cassette; dhfrllb-p15AoriV Brett et al., unpublished
  • a CF, cystic fibrosis.