Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaSource and/or reference
Strains
E. coliDH5aGibco BRL
P. aeruginosa
  CHAMucoid CF isolate 24
  CHA-D1 exsA::Gm mutant of CHA 2
  CF2, CF3, CF4, CF5, CF9, CF10,   CF15, RIE, K569, REN3, REN4Noncytotoxic CF isolates possessingexsA, exoS, exoT, and exoYgenes 3
  CF17Noncytotoxic CF isolate possessing exsA, exoS, exoT,exoY, and exoU genes 3
  CF6, CF11, CF12, REN0Cytotoxic CF isolates possessingexsA, exoS, exoT, and exoYgenes 3
Plasmids
 pUCP20Apr 25
 pDD2pUCP20-derived plasmid containing the exsAgene in the sense orientation 2
 pDD3pUCP20-derived plasmid containing the exsAgene in the inverse orientationThis work
 pUCP20-gfpmut3pUCP20 containing thegfpmut3 geneThis work
 pIA101* pUCP20-gfpmut3 with deletion of the plac −10 regionThis work
 pIApCpIA101*containing pexsC This work
 pIAX12pIA101*-derived plasmid containing thegfpmut3 gene under a constitutive promoter X12This work (2)
  • a Apr, ampicillin resistance.