Table 2.

Effect of repeated LPS injections on peritoneal leukocyte countsa

TreatmentbTotal leukocytes (106)No. of PMN (106)No. of mononuclear cells (106)
Naive1.7 ± 0.2<0.021.7 ± 0.2
1 × LPS4.5 ± 1.0c 3.7 ± 0.9d 0.8 ± 0.1
2 × LPS7.9 ± 4.3d 6.5 ± 3.7d 1.5 ± 0.6
3 × LPS23.3 ± 2.4d 17.4 ± 3.2d 5.9 ± 1.0d
  • a Mice were treated with serovar Abortus equi LPS (1 mg/kg i.p.) one to three times at 24-h intervals. Twenty-four hours after the last LPS injection, total and differential counts of peritoneal cells were performed. Saline injection had no effect on peritoneal cell numbers compared to those of naive mice. Data are expressed as means ± SD (n = 3).

  • b 1 ×, 2 ×, and 3 × LPS indicate one-, two-, and three-injection regimens.

  • c Significantly different from control (P < 0.05).

  • d Significantly different from control (P < 0.01).