Table 1.

Effect of IFN-γ on the intracellular growth ofL. monocytogenes in neural tissuea

Time (h) after infectionCFU (104/ml of lysate) of L. monocytogenes in samples treated with IFN-γ at the indicated U/ml:
0550
14.43.11.1b
69.34.41.1b
2425173.5b
  • a DRG cell cultures were incubated with recombinant IFN-γ 24 h before infection with L. monocytogenes at a multiplicity of infection of 2 bacterium per DRG cell. One hour after infection, cells were washed, gentamicin was added, and recombinant IFN-γ was replaced. Cells were washed and lysed 1, 6, or 24 h after infection, and L. monocytogenes CFU in the lysates were quantified. All conditions were used in triplicate. The results of one of three representative experiments are shown.

  • b Differences compared with the untreated group were significant ( P, <0.05 , as determined by the Studentt test).