Table 1.

Strains and plasmids used in this study

Strain or plasmid Genotype or relevant characteristicsReference or source
Strains
E. coliDH5 F (Φ80dlacZΔM15) Δ(lacZY AargF)U169 deoR recA1 endA1 hsdR17 (rK mK +),supE44 λ-thi-1 gyrA96 relA1 λ-CH616Gibco-BRL (7)
B. quintana ‘Oklahoma’
Plasmids
 pBBR1-MCS2 Kmr Mob Rep origin, Bartonella shuttle vector Michael Kovach (7)
 pFPV25 Source of promoterless GFP (gfpmut3a)Raphael Valdivia
 PJMBGFP PBBR1-MCS2 with a 970-bpHindIII fragment containing B. bacilliformisflagellin promoter (fla-pro) upstream ofgfpmut3a James M. Battitsti (2)