Table 1.

Induction of cytokines in C3H/OuJ macrophages stimulated with E. coli LPS or with P. gingivalis LPSa

CytokineLevel (pg/ml)b
Medium onlyE. coliK235 LPSP. gingivalis LPS
TNF-α (6 h)<2312,713 ± 3,456757 ± 62
IL-12 p40 + p70
 6 h<152,701 ± 127150 ± 32
 24 h<157,065 ± 438328 ± 25
IL-12 p70
 6 h<15143 ± 2<15
 24 h<15279 ± 20<15
  • a C3H/OuJ macrophages were cultured at a final density of 4 × 106/well in six-well plates and treated with 2 ml of medium only or medium containing 100 ng ofE. coli K235 LPS or P. gingivalis LPS per ml. Supernatants were harvested at 6 or 24 h and assayed by ELISA for TNF-α, total IL-12 (IL-12 p40 plus p70), or bioactive IL-12 p70 as described previously (29).

  • b Arithmetic mean ± standard deviation of duplicate samples.