Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotypeReference
E. colistrains
 HB101F′laqI q 3
 KB28 fimH gene from wild-type strain CI#1037
 KB53 fimH gene from wild-type strain CI#437
 KB91 fimH gene from commensal strain F-1837
 KB96 fimH gene from wild-type strain MJ2-237
 S1918F′laqI qΔfimB-H::kan 4
 MS72pMAS1 and pPKL115 in S1918This study
 MS174pMAS58 and pPKL115 in S1918This study
 MS202pMAS60 and pPKL115 in S1918This study
 MS206pMAS64 and pPKL115 in S1918This study
 PK799pPKL236 and pPKL115 in S1918This study
 PK800pPKL238 and pPKL115 in S1918This study
 PK802pPKL237 and pPKL115 in S1918This study
 PK803pPKL239 and pPKL115 in S1918This study
 TK130pTBK31 and pPKL115 in S1918This study
Plasmids
 pPKL4Wild-type fimgene cluster15
 pPKL115All fim genes exceptfimH 26
 pMAS1 fimH gene in pUC1932
 pMAS58Modified fimH gene (V28A, V56A, F71L, Y82H, T87A, V94A, W103R, S113G, V118A)This study
 pMAS60Modified fimH gene (V30L, G73E, S114R, N136Y, Q143L, V155G)This study
 pMAS64ModifiedfimH gene (G73E, L107F)This study
 pPKL236Modified fimH gene (W103R, S113G, V118A)This study
 pPKL237Modified fimH gene (V28A, V56A, F71L, Y82H, T87A V94A)This study
 pPKL238Modified fimH gene (S114R, N136Y, Q143L, V155G)This study
 pPKL239ModifiedfimH gene (V30L, G73E)This study
 pTBK31Modified fimH gene (G73E)This study