Table 2.

Active immunization of C3H/HeJ mice with recombinant pneumococcal proteins in Freund's adjuvant protects mice from a lethal challenge with a serotype 6B strain of pneumococci

AntigenMotifAccession no.Subcellular locationaDatabase similarityb (accession no.)ExptNo. of survivors/total no.
Active immunizationcSham immunization
Sp36SPase II AF291695 CW/MUnknown protein from Streptococcus agalactiae(AAD13797)19/100/10
210/100/10
Sp46Choline-binding protein AF291696 MEndo-β-N-acetylglucosaminidase (LytB) from S. pneumoniae (CAA 09078)16/100/10
27/100/10
Sp91Choline-binding protein AF291697 M1,4-β-N-acetylmuramidase (LytC) from S. pneumoniae(CAA08765)19/101/10
210/101/10
Sp101SPase II AF291698 CW/MUnknown protein fromBacillus anthracis (AAF13613)17/100/10
29/102/10
Sp128Cell wall anchor AF291699 CW/MCell wall-associated serine proteinase from S. pneumoniae (AAD48399)19/100/10
26/70/10
Sp130Cell wall anchor AF291699 CW/MCell wall-associated serine proteinase from S. pneumoniae (AAD48399)19/100/10
210/100/10
PspAControlCW/M110/103/10
210/100/10
  • a CW, cell wall fraction; M, membrane fraction.

  • b Homology searches were performed by comparing amino acid sequences against the nonredundant protein database using the BLAST algorithm at the National Center for Biotechnology Information. The most homologous amino acid sequence and its accession number are shown here.

  • c The number of survivors compared to that for the sham-immunized control was found to be statistically significant (P < 0.05) for all groups by using a two-sample log rank test.