TABLE 1

Mice and experimental design

Day of pregnancy (dpi)Mouse typeInfection statusNo. of miceNo. of pregnant micePregnancy rate (%)No. of implantation sites (mouse)aNo. of tissue samples used for histopathology (mouse)a,bNo. of tissue samples used for detection of no. of parasites (mouse)a,cNo. of tissue samples used for real-time RT-PCR analysis of mRNA (mouse)a,dNo. of serum samples used for detection of RANTES and IFN-γe
E6 (3)Wild typeUninfected441003 (1), 2 (2), 6 (3), 9 (4)1 (1), 1 (2), 3 (3), 4 (4)2 (1), 1 (2), 0 (3), 0 (4)f2 (1), 1 (2), 0 (3), 0 (4)f2f
Infected661009 (1), 9 (2), 7 (3), 10 (4), 9 (5), 8 (6)4 (1), 4 (2), 3 (3), 5 (4), 4 (5), 4 (6)5 (1), 5 (2), 4 (3), 5 (4), 5 (5), 4 (6)5 (1), 5 (2), 4 (3), 5 (4), 5 (5), 4 (6)6
CCR5−/−Uninfected4410010 (1), 6 (2), 9 (3), 10 (4)5 (1), 3 (2), 4 (3), 5 (4)5 (1), 3 (2), 0 (3), 0 (4)f5 (1), 3 (2), 0 (3), 0 (4)f2f
Infected661008 (1), 10 (2), 10 (3), 8 (4), 9 (5), 8 (6)4 (1), 5 (2), 5 (3), 4 (4), 4 (5), 4 (6)4 (1), 5 (2), 5 (3), 4 (4), 5 (5), 4 (6)4 (1), 5 (2), 5 (3), 4 (4), 5 (5), 4 (6)6
E8 (5)Wild typeUninfected221008 (1), 8 (2)4 (1), 4 (2)4 (1), 4 (2)4 (1), 4 (2)2
Infected6466.70 (1), 9 (2), 8 (3), 0 (4), 7 (5), 6 (6)0 (1), 4 (2), 4 (3), 0 (4), 4 (5), 3 (6)0 (1), 4 (2), 4 (3), 0 (4), 3 (5), 3 (6)0 (1), 4 (2), 4 (3), 0 (4), 3 (5), 3 (6)6
CCR5−/−Uninfected331008 (1), 9 (2), 8 (3)4 (1), 4 (2), 4 (3)4 (1), 5 (2), 4 (3)4 (1), 5 (2), 4 (3)3
Infected6583.311 (1), 0 (2), 9 (3), 11 (4), 9 (5), 7 (6)6 (1), 0 (2), 5 (3), 6 (4), 5 (5), 4 (6)5 (1), 0 (2), 4 (3), 5 (4), 4 (5), 3 (6)5 (1), 0 (2), 4 (3), 5 (4), 4 (5), 3 (6)6
E10 (7)Wild typeUninfected331009 (1), 6 (2), 9 (3)9 (1), 6 (2), 9 (3)9 (1), 6 (2), 9 (3)ND3
Infected51204 (1), 0 (2), 0 (3), 0 (4), 0 (5)4 (1), 0 (2), 0 (3), 0 (4), 0 (5)4 (1), 0 (2), 0 (3), 0 (4), 0 (5)ND5
CCR5−/−Uninfected3266.77 (1), 7 (2), 0 (3)7 (1), 7 (2), 0 (3)7 (1), 7 (2), 0 (3)ND2g
Infected7685.710 (1), 8 (2), 7 (3), 0 (4), 6 (5), 8 (6), 8 (7)10 (1), 8 (2), 7 (3), 0 (4), 6 (5), 8 (6), 8 (7)10 (1), 8 (2), 7 (3), 0 (4), 6 (5), 8 (6), 8 (7)ND7
  • a Numbers in parentheses denote the mouse identification number per experimental group. ND, no data.

  • b See Fig. 3.

  • c See Fig. 4.

  • d See Fig. 5.

  • e See Fig. 6.

  • f Because two of four uninfected mice in each group were added after experimental infection, only samples collected in the first experiment were analyzed.

  • g Because no implantation sites were detected in one of the three uninfected mice, this animal was removed from consideration.