Table 1.

Gentamicin does not inhibit intracellular parasitism of circulating phagocytesa

TreatmentNo. of bacteria/cellb% of extracellular GFP+ bacteriab, c% of GFP+ bacteria that are intracellularb, d
Extracellular GFPExtracellular GFP+Intracellular GFP+
None (control)3.33 ± 0.410.87 ± 0.271.08 ± 0.2318.3 ± 4.159.6 ± 4.5
Gentamicin2.88 ± 0.370.18 ± 0.051.20 ± 0.356.7 ± 1.085.8 ± 2.3
P0.820.0050.950.001<0.001
  • a Mice without (control) and with gentamicin-containing osmotic pumps were infected with L. monocytogenes NF-L512. Leukocytes were harvested 3 to 5 days postinfection, and the intracellular and extracellular bacteria were quantified by fluorescence microscopy.

  • b Results presented are the mean ± SEM for five or six animals in each group. P values are for control versus gentamicin-treated groups (Student's two-tailedt-test).

  • c Calculated as (extracellular GFP+bacteria/total extracellular bacteria) × 100.

  • d Calculated as (intracellular GFP+bacteria/total extracellular bacteria) × 100.