TABLE 2

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    N. gonorrhoeae
        FA1090Streptomycin-resistant WT strain80
        misS mutantFA1090 misS::kan insertion mutantThis study
        misR mutantFA1090 misR::erm insertion mutantThis study
    E. coli
        DH5α and TOP10Strains used for general cloning proceduresInvitrogen
        DY380DH10B derivative containing a defective λ prophage in which the red, bet, and gam genes are controlled by the temperature-sensitive λcI857 repressor81
Plasmids
    pGEM-T easyCloning vectorInvitrogen
    pKF20pGEM-T Easy containing the misS gene along with flanking sequencesThis study
    pUC18,KmRVector containing the kanamycin resistance cassette82
    pKF21pKF20 with the misS gene insertionally inactivated by a kanamycin resistance cassetteThis study
    pKF22pGEM-T Easy containing the misR gene along with flanking sequencesThis study
    pGCC3Vector containing the erythromycin resistance cassette83
    pKF23pKF22 with the misR gene insertionally inactivated by an erythromycin resistance cassetteThis study