TABLE 1

Significantly overrepresented S. Typhimurium genes with transposon insertions detected by TraDISb

Systematic IDaGene nameNo. of readsLog2 read ratioP valueDescription
InputOutput
SL1344_1088mdoH1,6611,825,51610.0185.2E−22Glucans biosynthesis glucosyltransferase H
SL1344_1087mdoG1,0171,092,8599.9345.30E−22Glucan biosynthesis protein G
SL1344_4050metL2,101255,2446.8586.02E−12Bifunctional aspartokinase II/homoserine dehydrogenase
SL1344_3345mreC13914,1585.8997.69E−11Rod shape-determining protein MreC
SL1344_0718sucA9210,1035.7321.63E−102-Oxoglutarate dehydrogenase E1 component
SL1344_3433cap1,12354,2575.4748.81E−09Septum formation protein Maf
SL1344_0124murF104,7095.4509.98E−16UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diaminopimelate–d-alanalanyl ligase
SL1344_0628mrdA395,5995.3581.24E−12Penicillin-binding protein 2
SL1344_1820zwf21912,9615.3562.86E−08Glucose 6-phosphate dehydrogenase
SL1344_4496deoB21610,6045.0825.07E−08Phosphopentomutase
SL1344_3954glnA67823,9244.9495.19E−07Glutamine synthetase
SL1344_3344mreD834,5834.6786.92E−09Rod shape-determining protein
SL1344_1622sapA1004,8114.6181.79E−07Peptide transport periplasmic protein
SL1344_2486yfgA1876,9364.6167.08E−07Cytoskeleton protein RodZ
SL1344_06301134,9684.5728.55E−08Ribosome-associated protein
SL1344_4489osmY3899,3904.2791.69E−06Hyperosmotically inducible periplasmic protein
SL1344_0665nagA1133,7944.1927.88E−07N-acetylglucosamine–6-phosphate deacetylase
SL1344_0629ybeA1112,8813.8201.45E−06(Pseudouridine1915-N3)-methyltransferase
SL1344_4081murB992,3473.6201.55E−06UDP–N-acetylenolpyruvoylglucosamine reductase
SL1344_3894cyaA5,79167,5523.5224.62E−04Adenylate cyclase
SL1344_3376sapG2703,3613.2266.53E−04trk system potassium uptake protein
SL1344_14741251,8713.1311.61E−71Putative multidrug efflux protein
SL1344_3939trkH1,71315,7803.1316.45E−03trk system potassium uptake protein TrkH
SL1344_1623sapB189093.0966.80E−70Dipeptide transport system permease protein
SL1344_2209861,1982.8035.59E−57Tail fiber assembly protein
SL1344_16843853,1052.7249.59E−54Putative regulatory protein
SL1344_3929tatC1,2238,3242.6711.35E−51sec-independent protein translocase protein
SL1344_4049metB1,76810,4962.5044.40E−02Cystathionine gamma-synthase
SL1344_04481311,2062.4995.01E−45Hypothetical protein
SL1344_1442dcp3722,5052.4649.45E−44Dipeptidyl carboxypeptidase II
SL1344_0443clpX5093,2272.4503.23E−43ATP-dependent clp protease ATP-binding subunit
SL1344_4011pfkA2681,9102.4493.64E−036-Phosphofructokinase
SL1344_2007cbiE1181,0412.3881.34E−03Precorrin-6Y C5,15-methyltransferase
SL1344_1693narK1601,2292.3548.11E−40Nitrite extrusion protein; MFS transporter
SL1344_3682rfaY4952,8922.3305.29E−39Lipopolysaccharide core biosynthesis protein
SL1344_32801471,1182.3024.84E−38Hypothetical protein
SL1344_2377nupC2041,3032.2067.05E−35Nucleoside permease NupC
SL1344_3927tatA4082,2252.1941.73E−34sec-independent protein translocase protein
SL1344_3726slsA1,2886,2222.1872.89E−34Hypothetical protein
SL1344_0091ksgA1871,1582.1321.63E−32Dimethyladenosine transferase (adenine1518-N6/adenine1519-N6)-dimethyltransferase
SL1344_3928tatB1,0134,5522.0632.06E−30sec-independent protein translocase protein
SL1344_0678ybfF1177932.0419.67E−30Putative esterase/lipase
SL1344_1624sapC475012.0321.84E−29Peptide transport system permease
SL1344_44244,35318,0082.0243.12E−29Type II restriction enzyme
SL1344_1236495022.0145.87E−29Putative MutT family protein
SL1344_1626sapF324302.0051.08E−28Peptide transport system ATP-binding protein
  • a Old locus tags from SL1344 annotation (GenBank accession: NC_016810).

  • b Significantly overrepresented S. Typhimurium genes with transposon insertions were detected by transposon-directed insertion site sequencing (TraDIS) following exposure of an S. Typhimurium SL3261 (SL1344 aroA mutant) saturating transposon insertion library to immune Malawian adult serum for 180 min. All genes with a log2 read ratio of >2 for output reads compared to input reads are included.