Table 1.

Strains used in this study

StrainRelevant characteristicsaReference or source
E. coli
 DH5αCloning host25
 CAB1K1 E. coli clinical isolateLaboratory collection
N. meningitidis
 NMBB:2B:P1.2,5:L2 (CDC8201085), parent strain for all following constructs28
 43 synD::Tn916, class I insertion30
 M7 synA::Tn916, class II insertion33
 700 ctrA::Tn916, class II insertion30
 ΩINT1Ω(Spr) cassette inserted into SnaBI site within intergenic regionThis study
 ΔΩINT1Same as ΩINT1 but with sequence between JS75 and JS74 primers deletedThis study
 ΩINT2Ω(Spr) cassette inserted intoEcoRI site 10 bp downstream of synA ATG start codonThis study
 ΔΩINT2Same as ΩINT2 but with sequence between JS94 and JS93 primers deletedThis study
 SA1 lacZ-erm cassette inserted intoSnaBI site within intergenic regionThis study
 SA2 lacZ-erm cassette inserted intoSnaBI site created within synA 339 bp from TTSThis study
 CA2 lacZ-ermC cassette inserted into SspI site within ctrA, 285 bp from TTSThis study
 SA286SA2 strain with 70 bp between primers JS56 and JS86 deleted, removing both DR and IRThis study
 CA286CA2 strain with same deletion as SA286This study
 SA293SA2 strain with 46 bp between primers JS56 and JS93 deleted, removing only DRThis study
 CA293CA2 strain with same deletion as SA293This study
 SA233SA2 strain with 20 bp downstream of synA ATG start codon deleted, removing only IRThis study
 CA233CA2 strain with same deletion as SA233This study
 SA247SSA2 strain with 5 bp (TTATA) mutated to CCGGG within DRThis study
 CA247CA2 strain with the same mutation as SA247This study
 988 lacZ-ermC cassette inserted into chromosomal locus 120A1This study
 SA295 syn promoter fragment containing 95 bp upstream of TTS fused to lacZ-ermCcassette and integrated into 120A1 locusThis study
  • a TTS, transcriptional start site; DR, direct repeat; IR, inverted repeat.