Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
H. influenzae
 DB117 H. influenzae Rd strain, rec-1 29
 N182Wild-type NTHI strain5
 N182.11Isogenic N182hgbA mutant containing an Ω chloramphenicol resistance cartridge inserted at the XhoI site in the hgbAgeneThis study
 N182.12Isogenic N182 hgbBmutant containing an erythromycin resistance cartridge inserted at theNcoI site in the hgbB geneThis study
 N182.13Isogenic N182 hgbC mutant containing a kanamycin resistance cartridge inserted at the XhoI site in the hgbC geneThis study
 N182.14Isogenic N182hgbA hgbB mutantThis study
 N182.15Isogenic N182 hgbA hgbC mutantThis study
 N182.16Isogenic N182 hgbB hgbC mutantThis study
 N182.17Isogenic N182 hgbA hgbB hgbCmutantThis study
 N182.18Isogenic N182 hgbA hgbC mutant constructed by introducing the hgbAmutation into N182.13This study
 N182.100Isogenic N182 mutant containing a spectinomycin resistance cartridge inserted at theSspI site in the hxuC geneThis study
 N182.114Isogenic N182 hgbA hgbB hxuCmutantThis study
 N182.115Isogenic N182 hgbA hgbC hxuC mutantThis study
 N182.116Isogenic N182hgbB hgbC hxuC mutantThis study
 N182.117Isogenic N182 hgbA hgbB hgbC hxuCmutantThis study
 N182.217Isogenic N182 hgbA hgbB hgbC mutant with a spectinomycin resistance cartridge inserted into the SspI site in the tonB geneThis study
 N182.317Isogenic N182 hgbA hgbB hgbC mutant with a spectinomycin resistance cartridge inserted into the ScaI site in the tdhA geneThis study
E. coli DH5αHost strain used for cloning experiments26
Plasmids
 pLDC300Previously designated pHgbA-FP; expresses β-lactamase–HgbA fusion protein3
 pLDC400Previously designated pHgbB-FP; expresses β-lactamase–HgbB fusion protein3
 pLDC500Previously designated pHgbC-FP; expresses β-lactamase–HgbC fusion protein3
 pLDC301pLDC300 with an Ω chloramphenicol resistance cartridge inserted at the XhoI site in the partialhgbA ORFThis study
 pLDC401pLDC400 with an erythromycin resistance cartridge inserted at the NcoI site in the partial hgbB ORFThis study
 pLDC501pLDC500 with a kanamycin resistance cartridge inserted at the XhoI site in the partial hgbC ORFThis study
 pLDC600pCR-Blunt II-TOPO containing the N182tonB geneThis study
 pLDC601pLDC600 with a spectinomycin resistance cartridge inserted into the SspI site in the tonB ORFThis study
 pLDC700pCR-Blunt II-TOPO containing a 2-kb incomplete N182hxuC ORFThis study
 pLDC701pLDC700 with a spectinomycin resistance cartridge inserted into the SspIsite in the partial hxuC ORFThis study
 pLDC800pCR-Blunt II-TOPO containing a 1.8-kb incomplete N182tdhA ORFThis study
 pLDC801pLDC800 with a spectinomycin resistance cartridge inserted into the ScaI site in the partial tdhA ORFThis study
 pGJB103Cloning vector capable of replication in E. coli and H. influenzae 1
 pHXUC-1pGJB103 containing the 2.4-kb N182 hxuC geneThis study