Table 2.

Specific antibody (IgG and IgM) and opsonophagocytic activity of serum from N. meningitidis disease study subjects

Patient no. (sex)Infecting N. meningitidis strainaMAb B5 reactivityb (immunotype)Age (mo)ELISA titer, AU/mlcMean % of opsonophagocytic activityd (95% CI)
L2 galE (IgG)L3galE (IgG)L2 galE(IgM)L3 galE (IgM)
AcuteConvalescentAcuteConvalescentAcuteConvalescentAcuteConvalescentAcuteConvalescent
1 (F)B (PCR)nk111.01.350.66.02.13.21.06.610.5 (5–15.9)47.5* (29–65.9)
2 (F)B:NT:P1.14+ (L1)481.22.712.912.66.98.06.411.221.0 (21–21)69.8* (57.5–82)
3 (F) N. meningitidisisolatednk1087.09.97.130.38.7>102.86.535.3 (32.5–38)59.9* (56.7–63)
4 (M)B:15:P1.7+ (L3,7)1682.04.81.013.7>10>102.29.322.5 (17–28)53.8* (50–57.5)
5 (F)B:NT:P1.15nk221.9>100.656.54.5>10nk>10.07.4 (7.1–7.7)22.2* (17.9–26.5)
6 (M)C:2a:P1.5± (L2)561.6>104.67.42.310.42.310.425.6 (24.7–26.5)39.8* (38.2–41.3)
7 (M)B (serology)nk828.76.52.13.82.53.63.43.015.0 (14–16)13.9 (12.4–15.3)
8 (M)Clinical diagnosisnk84.36.32.0>104.06.60.33.314.3 (14.1–14.5)29.9 (28–31.8)
9 (M)C:2a:P1.5nk951.5>105.9>10>10>101.35.123.3 (22.1–24.6)15.4 (15.2–15.6)
  • a Patient 1 had positive PCR on cerebrospinal fluid (CSF) (serogroup B), but no isolate grew; patient 3 had cloudy CSF and penicillin-resistant N. meningitidis, but the isolate was nontypeable; patient 7 had antibodies to serogroup BN. meningitidis, but no isolate was cultured; patient 8 had a definite clinical diagnosis of meningococcus, but no group, type, or subtype is available.

  • b nk, not known.

  • c Sera were diluted 1:50 in 1% BSA–PBS, andgalE units were determined from adult pooled serum standard curve.

  • d Sera were diluted 1:2 in final buffer with addition of 5 μl of heterologous human complement. *, significant difference between paired sera ( P < 0.05 , two-tailedt test).