Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsReference or source
P. multocida X-73Serogroup A: serotype 1 chicken isolate; reference strain 23
PmIVET clones
 MHS6X-73 harboring three noncontiguous X-73 genomic Sau3AI fragments, 355, 253, and 127 bp, in pMKΩThis study
 MHS7X-73 harboring three noncontiguous X-73 genomic Sau3AI fragments, 143, 149, and 967 bp, in pMKΩThis study
 MHS17X-73 harboring two noncontiguous X-73 genomic Sau3AI fragments, 339 and 191 bp, in pMKΩThis study
 MHS23X-73 harboring a 604-bp X-74 genomic Sau3AI fragment in pMKΩThis study
 MHS25X-73 harboring four noncontiguous X-73 genomicSau3AI fragments, 94, 98, 351, and 144 bp, in pMKΩThis study
 MHS27X-73 harboring a 465-bp X-73 genomicSau3AI fragment in pMKΩThis study
 MHS30X-73 harboring three noncontiguous X-73 genomic Sau3AI fragments, 123, 276, and 387 bp, in pMKΩThis study
 MHT6X-73 harboring a 267-bp X-73 genomic Sau3AI fragment in pMKΩThis study
 MHW32X-73 harboring three noncontiguous X-73 genomic Sau3AI fragments, 666, 267, and 129 bp, in pMKΩThis study
 MHW39X-73 harboring two noncontiguous X-73 genomic Sau3AI fragments, 198 and 909 bp, in pMKΩThis study
 MHX6X-73 harboring two noncontiguous X-73 genomic Sau3AI fragments, 431 and 549 bp, in pMKΩThis study
 MHY6X-73 harboring four noncontiguous X-73 genomic Sau3AI fragments, 171, 1,111, 158, and 876 bp, in pMKΩThis study
 MHY29X-73 harboring two noncontiguous X-73 genomic Sau3AI fragments, 119 and 261 bp, in pMKΩThis study
 MHY40X-73 harboring six noncontiguous X-73 genomic Sau3AI fragments, 444, 295, 465, 092, 021, and 123 bp, in pMKΩThis study
 MHZ12X-73 harboring a 66-bp X-73 genomic Sau3AI fragment in pMKΩThis study
Plasmids
 pUC4-KIXXAmpr, Kanr, ColE1 origin 51
 pUCΩAmpr, Sper, Strr, ColE1 origin 46
 pPMK14.9-kb Kanr,P. multocida origin 6
 pPBA8442.0-kb BamHI SPE/STR cassette of pUCΩ cloned into BglII-digested pPMK1, removing kangene promoterThis study
 pMKΩSPE and STR selection in vitro; 6.5 kb Expand PCR product generated using primers BAP-03 and BAP-04 from pPBA844 template, digested with BamHI and religated on itself; used for selection of in vivo-expressed genes in PmIVETThis study