Table 3.

Phenotypes of putative ST mutant strains after orogastric and i.p. infection of BALB/c mice

Strain/genotyped (phenotype)Orogastric infection with 2 × 109 bacteriaaMean log difference (log CFU of ST strain − log CFU of WT) after coinfection with equal mixture of WT and mutant Y. pseudotuberculosis (Pe)
Orogastricb (n = 5)i.p.c (n = 5)
No. dead/no. infectedDays until deathCecumPPMLNSpleen
YPIIIpYV (wild type)6/64, 4, 5, 6, 7, 11
425/yscH (type III secretion)0/3−2.20 ± 0.27 (<0.0009)
426/yscU (type III secretion)0/3
431/yscB (type III secretion)0/3
542/yscL (type III secretion)0/3
429/lcrR (regulator of type III secretion)0/3−2.01 ± 1.62 (0.05)−2.071 ± 1.67 (0.05)−1.40 ± 0.64 (0.008)−0.75 ± 0.18 (<0.0009)
498/lcrR (regulator of type III secretion)0/3−2.68 ± 0.59 (<0.0009)
427/ddhC (O-Ag biosynthesis)0/3−1.51 ± 0.12 (<0.0009)
496/wzx (O-Ag biosynthesis)2/312, 16−2.71 ± 1.07 (0.004)−2.42 ± 0.03 (0.032)−2.67 ± 0.64 <0.0009−2.15 ± 0.83 (0.0044)
543/gmd (O-Ag biosynthesis)4/67, 9, 21, 24−2.97 ± 1.5 (0.027)−2.02 ± 0.25 (0.005)−2.14 ± 0.63 (0.027)−1.94 ± 0.50 (0.0020)
494/inv (invasin)2/37, 7−1.63 ± 0.26 (0.001)−1.41 ± 1.36 (0.080)−2.15 ± 0.7 (0.003)0.03 ± 0.34 (0.855)
497/sufl (suppressor offtsI)0/3−1.70 ± 0.78 (0.008)
499/cls (cardiolipin synthesis)2/312, 15−3.42 ± 1.57 (0.004)−3.31 ± 0.67 (0.032)−2.16 ± 0.72 (0.001)−1.21 ± 0.29 (0.0007)
500/ksgA (kasugamycin resistant)1/324−0.59 ± 0.39 (0.027)−0.76 ± 0.68 (0.065)−2.14 ± 0.63 (0.027)−0.74 ± 0.36 (0.011)
4283/36, 6, 10
4303/35, 9, 10
4934/68, 8, 10, 12−1.00 ± 1.24 (0.204)−0.41 ± 1.01 (0.47)−0.37 ± 1.50 (0.65)
4953/36, 7, 80.40 ± 1.14 (0.47)−0.08 ± 0.9 (0.85)0.33 ± 1.3 (0.71)0.07 ± 0.49 (0.76)
5443/37, 8, 9
5453/36, 8, 8
  • a Eight-week-old mice were infected with 2 × 109 bacteria and observed until death or for 40 days.

  • b Mice were infected with an equal mixture of 2 × 109 WT Y. pseudotuberculosis and the indicated ST strain. After 5 days, the numbers of WT and ST bacteria in the cecum, PP, and MLN were determined.

  • c Mice were infected i.p. with an equal mixture of 106 WT Y. pseudotuberculosis and the ST mutant. After 3 days, the numbers of WT Y. pseudotuberculosis and ST bacteria in the spleens was determined.

  • d The insertions within the yscH, yscB, lcrV, ddhC, wzx, gmd, cls, and ksgA genes may be polar on downstream open reading frames.

  • e From a two-tailed, paired Student'st test.