Table 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerGenotype, description, or sequenceaReference or source
Strains
E. coli
  DH5αφ80 Δ(lacZ)M15 Δ(argF-lac)U169 endA1 recA1 hsdR17 (rKmK+) deoR thi-1 supE44 gyrA96 relA156
S. aureus
  8325-4Wild-type strain cured of prophagesLab stock
  RN4220Restriction-deficient transformation recipientLab stock
  MJH001perR::kanThis study
  MJH002ahpC::pAZ106ahpC+This study
  MJH003bcp::pAZ106bcp+This study
  MJH004ftn::pAZ106ftn+This study
  MJH005fur::pAZ106fur+This study
  MJH006katA::pAZ106katA+This study
  MJH007mrgA::pAZ106mrgA+This study
  MJH008perR::pAZ106perR+This study
  MJH009trxB::pAZ106trxB+This study
  ST16katA::Tn917-LTV1This study
  SPW1sodA::Tn917-LTV165
  MJH102perR::kan ahpC::pAZ106 ahpC+This study
  MJH103perR::kan bcp::pAZ106 bcp+This study
  MJH104perR::kan ftn::pAZ106 ftn+This study
  MJH105perR::kan fur::pAZ106 fur+This study
  MJH106perR::kan katA::pAZ106 katA+This study
  MJH107perR::kan mrgA::pAZ106 mrgA+This study
  MJH108perR::kan perR::pAZ106This study
  MJH109perR::kan trxB::pAZ106 trxB+This study
  MJH408perR::kan pMAL34 (perR+)This study
  MJH4188325-4 pMAL34 (perR+)This study
Plasmids
 pAZ106Promoterless lacZ erminsertion vector16
 pGem3Zf(+)General cloning vectorPromega
 pAUL-ATemperature-sensitiveerm integrational shuttle vector15
 pCU1E. coli-S. aureus cat shuttle vector7
 pMAL5pGem3Zf(+) containing a 2-kb perR fragment with engineered ClaI siteThis study
 pMAL7pMAL5 containing a kancassette in ClaI site.This study
 pMAL83.5-kbBamHI-HindIIIperR::kan fragment from pMAL7 in pAUL-AThis study
 pMAL91.08-kb OL7–OL11 perRpromoter-containing fragment in pAZ106This study
 pMAL100.6-kb OL13–OL14 fur promoter-contaiing fragment in pAZ106This study
 pMAL111.2-kb OL15–OL16katA promoter-containing fragment in pAZ106This study
 pMAL121.2-kb OL17–OL18 ahpCF promoter-containing fragment in pAZ106This study
 pMAL131.1-kb OL19–OL20mrgA promoter-containing fragment in pAZ106This study
 pMAL280.6-kb OL53–OL54 bcp promoter-containing fragment in pAZ106This study
 pMAL291.4-kb OL60–OL61ftn promoter-containing fragment in pAZ106This study
 pMAL301.4-kb OL62–OL63 trxB promoter-containing fragment in pAZ106This study
 pMAL340.9-kb OL82–OL83perR gene-containing fragment in pCU1This study
Primers
 OL7AATTGGATCCCATGGTTTGCAACGGGTGThis study
 OL8CCGGAAGCTTATCCTGAGCCAGGATCAAACTCTCCATThis study
 OL9GAATCAATTGCATCGATTGCGACAAGCAGGCGThis study
 OL10CGCCTCCTTGTCGCAATCGATGCAATTGATTCThis study
 OL11CCAGAATTCGAATCGACTTGATGAGTCTCCATATGThis study
 OL12AATTGGATCCTGTAATGGTTTGCCACTTTGCAGThis study
 OL13CCAGAATTCAGTAGCTTCGCGTTGTGGCGTTAGCThis study
 OL14AATTGGATCCATTACCAAACGGTGAAACGTCThis study
 OL15CCAGAATTCAACGCAGCTTGTTCAACATCCThis study
 OL16AATTGGATCCGACCACAATGCCCAATACAACCThis study
 OL17CCAGAATTCTGAATGTACCACGTTGAGCTAACThis study
 OL18AATTGGATCCATGGTAAGCGTGGCTTGGCTGCThis study
 OL19CCAGAATTCTACATCATCGCCAGCATTACCThis study
 OL20AATTGGATCCATGGCAATTTCGTGTCGCGAGGThis study
 OL53GGTTGGATCCAATTTCTAATTCAGTCGGTGTACCThis study
 OL54CCAGAATTCTAAATTGTCTCTAAAGTCACAAGCThis study
 OL60AGAAGGATCCGCGTTATAAGCGTTAAAGTCACThis study
 OL61AGCAGAATTCTGCATGTGCACCTCTGTCGThis study
 OL62AGAAGGATCCAGAACTGATTACGATTGGTAGThis study
 OL63AGCAGAATTCCTGCCAAATACTTTCCGTCATCThis study
 OL82AGAGGATCCACAGCGCATATAACTGGTAATGThis study
 OL83CCAGAATTCCTTATACTCACTTTATGGATAGThis study
 PEX2AGCTCCATGGTCTGACGCTCAGTGGAACGAAAACTCThis study
 PEX3CAGTAGCTTCGCGTTGTGGCGTTAGCThis study
 PEX4GGTGTAATTCTTACGCCTGCTTGTCGCThis study
 PEX5TCTCGGTCTGATACTGGATGCCThis study
 PEX6TTACAACATCTTGTTGATTACTCThis study
 Tn1GGAACGCCGTCTACTTACAAGCAGCThis study
 Tn2CTCACAATAGAGAGATGTCACCGTCThis study
  • a In primer sequences, the restriction sites are underlined.