Table 1.

PCR primers and amplification conditions used in this study

PrimerSequenceAmplification conditions (temp [°C], time [s])Product length (bp)
DenaturingAnnealingExtension
sfpA-U5′-AGCCAAGGCCAAGGGATTATTA-3′94, 3059, 6072, 60440
sfpA-L5′-TTAGCAACAGCAGTGAAGTCTC-3′
sfpDG-U5′-GATGCTGGCACCGAAGTCAA-3′94, 3057, 6072, 601,682
sfpDG-L5′-TCCTGCAGCATCCAAGTCAC-3′
wprom-35′-GCTGGACGCCGGTGCTTATT-3′94, 3061, 6072, 1501,900
wprom-45′-CCAGATTGCCGCTCGCTTCAG-3′
sfpG-UApa5′-CCCGGGCCCGGCTGCGCTGCTGGTGAG-3′94, 1555, 3068, 180a 3,162
sfpG-LApa5′-CCCGGGCCCTCATCTGCCGGTCCCTTAC-3′
  • a This PCR was performed using the Expand High Fidelity PCR system. From cycle 11 to 30 the extension time was prolonged 5 s with each cycle. The PCR was completed by a final elongation step of 7 min at 72°C.