Table 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
P. aeruginosa
 PAKClinical isolate of wild-type invasive strainD. Bradley
 PA103Clinical isolate of wild-type cytotoxic strain22
 PAKexsAPAK with chromosomal disruption of theexsA locus; Spr Smr14
 PAKrpoSPAK with chromosomal disruption of therpoS locus; Spr SmrThis study
 PAKfliCPAK with chromosomal disruption of thefliC locus; GmrR. Ramphal
 PAKexoSPAK with chromosomal disruption of theexoS locus; Spr Smr19
 PAKexoSexoTPAK with chromosomal disruption of theexoS and exoT loci; SprSmr Gmr19
 PA103exoUPA103 with chromosomal deletion of theexoU locus11
 PA103exoUexoTPA103-ΔexoU with chromosomal disruption of the exoT locus; Tcr33
Plasmids
 pCR2.1-TOPOPCR cloning vector; Apr KmrInvitrogen
 pDN19lacΩPromoterless lacZ fusion vector; Spr Smr Tcr32
 pFLAG-CTCFLAG peptide fusion vector; AprSigma
 pHW0004exoT gene derived from PAK in pUCP18; AprThis study
 pHW0005PAK exoSpromoter fused to promoterless lacZ in pDN19lacΩ; Spr Smr TcrThis study
 pHW0006PAK exoT promoter fused to promoterlesslacZ in pDN19lacΩ; Spr SmrTcrThis study
 pHW0015exoS gene derived from PAK in pUCP18; AprThis study
 pHW0017exoT gene derived from PA103 in pUCP18; AprThis study
 pHW0018PA103 exoTpromoter fused to promoterless lacZ in pDN19lacΩ; Spr Smr TcrThis study
 pHW0027exoT-FLAG of PAK in pUCP18; AprThis study
 pHW0028exoT-FLAG of PA103 in pUCP18; AprThis study
 pHW0029exoS-FLAG of PAK in pUCP18; AprThis study
 pUCP18Broad host range shuttle vector; Apr30
 pUCPexoSexoS gene derived from 388 in pUCP18; Apr21
 pUCPexoTexoT gene derived from 388 in pUCP18; Apr36
 pUCPexoSE381AexoS of pUCPexoS mutated into E381A; Apr34
  • a Phenotypes: Apr, ampicillin resistance marker; Kmr, kanamycin resistance marker; Gmr, gentamicin resistance marker; Spr, spectinomycin resistance marker; Smr, streptomycin resistance marker; Tcr, tetracycline resistance marker.