Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or phenotypeaSource or reference
Strains
A. caviae
  Sch3Clinical isolate54
  Sch3NSch3, spontaneous NalrThis study
  AAR269flaA::KmrThis study
  AAR27flaB::KmrThis study
  AAR31flaA::Cmr,flaB::KmrThis study
  AAR150flaG::KmrThis study
  AAR59flaH::KmrThis study
  AAR8flaJ::KmrThis study
E. coli
  CC118λpirΔ(ara leu)7697 araD139 ΔlacZX74 glaE glaK phoA20 thi-1 rspE rpoB(Rfr) argE(Am)recA1αpir+17
  S17-1λpirhsdR, pro, recA, RP4-2 in chromosome Km::Tn7 (Tc::Mu) λpir, Tpr Smr41
  XL1-BlueendA1 recA1 hsdR17 supE44 thi-1 gyrA96 relA1 lac [F′ proAB laclqZΔM15 Tn10(Tcr)]Stratagene
Plasmids
 pUC19High-copy-number cloning vector, MCS, AmprGibco-BRL
 pUTmini-Tn5Cmbla, ori R6K,mob RP4, tnp gene of Tn5-IS50r that lacks NotI site, MCS of M13tg131; 8.7 kb, Apr Cmr9
 pUC4-KIXXSource of Tn5-derived nptIIgene (Kmr)Pharmacia
 pBBR1MCSBroad-host-range vector; IncP, W, and -Q; Col E1; and p15A compatible, containing pBluescript II (KS)- lacZα-polylinker, Cmr32
 pBBR1MCS-3Broad-host-range vector; IncP, -W, and -Q; Col E1; and p15A compatible, carrying Tcr31
 pKNG101ori R6K,mob RK2, strAB sacBR, 6.8 kb; Smr21
 pARP1pUC19 with a 900-bp flaPCR insertThis study
 pARP2pBBR1MCS with a 3.2-kbSacI insert of A. caviae Sch3N DNAThis study
 pARP126pBBR1MCS with a 3.6-kb SacI insert ofA. caviae Sch3N DNAThis study
 pARA1001.5-kbXbaI-SmaI fragment containing flaA from pARP2, inserted at the pKNG101This study
 pARA1101.4-kb Kmr cassette from pUC4-KIXX inserted at the PvuII site of flaA in pARA100This study
 pARA1203.7-kb end-filledHindIII fragment from pUTmini-Tn5Cm, inserted at the PvuII site in pARA100This study
 pARB2001.2-kb SacI-HincII fragment containing flaB from pARP126 in pUC19This study
 pARB2101.8-kb KmrBamHI-SmaI fragment from pUC4-KIXX inserted at the BglII-EcoRV sites of flaB in pARB200This study
 pARB2203.7-kb CmrEcoRI fragment of pUTmini-Tn5Cm inserted at theEcoRI site of pARB210This study
 pARB2302.2-kbBamHI fragment from pARB220 inserted at the BamHI site of pKNG101This study
 pARP4003.6-kb SacI fragment from pARP126 inserted into pUC19This study
 pARG4101.4-kb KmrSmaI fragment from pUC4KIXX inserted at the StuI site of flaG in pARP400This study
 pARG4204.2-kb SalI fragment from pARG410 inserted at the SalI site of pKNG101This study
 pARH5002.2-kb SacI-StuI fragment from pARP400 inserted at SacI-SmaI site on pUC19.This study
 pARH5101.4-kb KmrSmaI fragment from pUC4KIXX inserted at the SmaI site of flaH pARH500This study
 pARH5203.6-kbSacI-BamHI fragment from pARH510 inserted atSacI-BamHI sites of pKNG101This study
 pARH5303.7-kb CmrBamHI fragment from pUTmini-Tn5Cm inserted at the BamHI site of pARH520This study
 pARJ6001.4-kb KmrSmaI fragment from pUC4KIXX inserted at BalI site of flaJ in pARH500This study
 pARJ6103.6-kbSacI-BamHI fragment from pARJ600 inserted at theSacI-BamHI sites of pKNG101This study
 pARJ6203.7-kb CmrBamHI fragment from pUTmini-Tn5Cm inserted at the BamHI site of pARJ610This study
 pARP12641.8-kbNaeI-SacI fragment from pARP2 inserted at theSmaI-SacI site of pBBR1MCS-3This study
 pARP12653.6-kb SacI insert from pARP126 inserted in the SacI site of pBBR1MCS-3This study
 pARP12663.6-kb SacI insert from pARP126 inserted in the SacI site of pARP1264This study
  • a Phenotype abbreviations are as follows: resistance (r); Amp (ampicillin); Cm (CHL); Km (KAN); Nal (NAL); Sm (STR); and Tc (tetracycline). MCS, multiple cloning site.