Table 2.

Specific IFN-γ secretion after vaccination with plasmid DNA

ImmunogenAntigen for in vitro stimulationa
P1P3P4Th38-kDaPPD
pXJ38++++++++++++++
pP3+++++
vTh+/−++
vTh+pP3++/−++++++++++
pThP3+++++++
  • a Specific IFN-γ secretion was measured as IFN-γ of spleen cells of mice immunized with immunogen −IFN-γ secretion of spleen cells of mice immunized with control vector. Five million spleen cells from DNA-immunized mice were incubated with the indicated antigens for 72 h. −, <50 pg/ml +/−, 50 to 100 pg/ml; +, 100 to 250 pg/ml; ++, 250 to 500 pg/ml; +++, 500 to 1,000 pg/ml; ++++, 1,000 to 1,500 pg/ml.