Table 1.

CTL responses after immunization with plasmid DNA

Immunogen% Specific lysis at T:E ratioa
1:801:401:20
pXJ3850 ± 12***44 ± 15***36 ± 16***
Control vector15 ± 811 ± 66 ± 3
pP312 ± 28 ± 17 ± 4
Control vector18 ± 1113 ± 97 ± 4
vTh18 ± 612 ± 49 ± 3
Control vector14 ± 510 ± 56 ± 3
vTh+pP324 ± 10**16 ± 8*9 ± 5
Control vector12 ± 57 ± 45 ± 3
pThP312 ± 610 ± 36 ± 4
Control vector12 ± 67 ± 45 ± 3
  • a CTL responses (percentage of specific lysis) measured after in vitro restimulation of the in vivo-primed spleen cells with mitomycin-treated EX-38 cells are shown. The effector cells (E) were incubated with EX-38 target cells (T) in a classical51C release assay. Results are presented as means ± standard deviations of three to eight independent experiments. Lysis of EL-4 cells (T) as the negative control was <4%. Comparisons were statistically significant compared to control vector in the same experiments at P < 0.0005 (***), P < 0.025 (**), P < 0.05 (*) according to Student'st-test.