Table 3.

Analysis of EHEC strains for A/E lesion formation

Strain, serotype, sourceA/E lesions after 6 haAdherence phenotypebEspD secretion (MEM)c
ZAP001, O157, human5LA++
ZAP004, O157, human3DA++
ZAP008, O157, human3DA++
ZAP010, O157, human4LA+++
ZAP058, O157, human9LA+++
ZAP065, O157, human2DA+/−
ZAP088, O157, human5LA++
ZAP028, O157, bovine3DA+/−
ZAP032, O157, bovine2DA+
ZAP034, O157, bovine3DA+/−
ZAP038, O157, bovine2DA+/−
ZAP041, O157, bovine2DA+/−
ZAP052, O157, bovine4LA++
ZAP054, O157, bovine1DA+/−
ZAP021, O26, bovine8LA+++
ZAP078, O26, human7LA+++
  • a Actin rearrangement around the site of attached bacteria was detected by FAS as described in Materials and Methods and previously (26, 31). Results are representative of monolayers and are shown as the number showing actin rearrangement per 10 HeLa cells when viewed at a magnification of ×1,000.

  • b Only strains exhibiting LA can be considered as forming microcolonies (29).

  • c Ability of strains to secrete EspD in MEM, as detected by Western blotting (Fig. 2 and 3).