Table 4.

Production of proinflammatory cytokines IL-1β and TNF-α by J774-A1 cells after AIEC LF82 infection

Gentamicin treatment period (h)IL-1β concn (pg/ml)ain:TNF-α concn (pg/ml)b
Uninfected cellsUninfected lysed cellsAIEC LF82- infected cellsS. flexneri M90T infected cellsUninfected cellsAIEC LF82- infected cellsLPS-stimulated cells
1 <10 (0%)97.0 ± 2.8 (100%) <10 (0%)40.5 ± 0.7 (41.7%)214 ± 48 99 ± 6 110 ± 5
24 <10 (0%) ND <10 (0%) ND 302 ± 133,533 ± 111 1,300 ± 86
  • a J774-A1 cells were prestimulated with LPS and then infected with S. flexneri M90T or AIEC LF82. Medium containing gentamicin was added, and IL-1β concentrations in culture supernatant were measured by ELISA at the indicated times of gentamicin treatment. Results are means ± standard deviations from two independent experiments performed in duplicate, expressed in picograms per milliliter and as percentage of total IL-1β. ND, not determined.

  • b J774-A1 cells were exposed to AIEC LF82 or to LPS for 2 h. Medium containing gentamicin was added, and TNF-α concentrations in culture supernatant were measured by ELISA at the indicated times of gentamicin treatment. Results are means ± standard errors of the means of two independent experiments performed in duplicate, expressed in picograms of secreted TNF-α per milliliter.