Table 3.

Enumeration of apoptotic and necrotic J774-A1 macrophages after bacterial infection or treatment with gliotoxin

% Apoptotic cellsa% Necrotic cellsa
8 h postinfection24 h postinfection8 h postinfection24 h postinfection
Untreated cells 10.1 ± 0.911.2 ± 1.2 1.6 ± 1.1 2.0 ± 0.7
AIEC LF827.8 ± 0.2 10.0 ± 1.6 1.4 ± 0.6 4.0 ± 1.4
Gliotoxin (5 μM) 36.6 ± 1.0 ND4.5 ± 0.4 ND
S. flexneriM90T 26.6 ± 2.8 19.5 ± 4.0 5.0 ± 1.212.4 ± 1.8
Serovar Cholereasuis 25.0 ± 2.328.0 ± 0.8 2.0 ± 1.2 5.5 ± 2.6
K-12 C60010.9 ± 2.5 11.7 ± 0.4 1.1 ± 1.10.9 ± 0.0
  • a Mean percentages ± standard errors of the means of apoptotic or necrotic cells visualized by using the annexin V-FITC assay (Sigma). The percentage of stained cells was scored by analysis of 100 cells of three independent experiments in duplicate. ND, not determined.