Table 2.

Effects of PvdS on pyoverdine production and proteolytic and elastolytic activitiesa

Strain/plasmid (presence of IPTG)Pyoverdine concn (M)Protease activity (ΔOD520/h/ml)Elastase activity (ΔOD495/h/ml)
PAO1/pVLT3156 (7)2.9 (1.0)0.042 (0.007)
PAO1/pPVD31 (−IPTG)50 (12)2.9 (0.2)0.044 (0.012)
PAO1/pPVD31 (+IPTG)130 (25)10.0 (2.0)0.066 (0.008)
PAO1 ΔpvdS/pVLT31NDb1.35 (0.34)0.021 (0.007)
PAO1 ΔpvdS/pPVD31 (−IPTG)0.5 (0.7)1.35 (0.32)0.023 (0.006)
PAO1 ΔpvdS/pPVD31 (+IPTG)21 (4)2.3 (0.038)0.061 (0.002)
  • a Standard deviation for three independent experiments is given in parentheses.

  • b ND, not detected.