Table 2.

Absence of LDH release by J774-A1 cells infected with AIEC strain LF82

Gentamicin treatment period (h)LDH activitya (% cytotoxicityb) at an MOI of:
10100
AIEC LF82K-12 C600S. flexneri M90TSerovar CholeraesuisAIEC LF82K-12 C600S. flexneri M90TSerovar Choleraesuis
1 20.8 ± 4.7 (2.2)16.1 ± 4.2 (0.4)  43.4 ± 15.6 (10.8)  27.0 ± 7.0 (4.6) 19.8 ± 4.8 (1.9) 21.7 ± 4.4 (2.6) 82.8 ± 19.2 (25.7)  29.5 ± 4.5 (5.5)
422.2 ± 5.2 (2.8) 23.2 ± 3.1 (3.2)  83.8 ± 37.1 (26.8)  31.0 ± 1.0 (6.1) 19.8 ± 5.2 (1.9)23.0 ± 5.7 (3.1) 131.5 ± 32.0 (44.2)  56.5 ± 2.5 (15.89)
8 29.5 ± 4.5 (5.5) 24.5 ± 9.5 (3.6)177.0 ± 29.5 (61.6)  35.5 ± 13.5 (7.8) 26.0 ± 8.0 (4.2) 27.5 ± 2.5 (4.8) 202.0 ± 5.0 (70.9)125.5 ± 61.5 (41.9)
24 41.8 ± 5.3 (10.2)43.8 ± 9.6 (10.9) ND 148.5 ± 0.4 (50.6) 42.8 ± 3.8 (10.6) 42.3 ± 4.4 (10.4)ND 208.5 ± 11.5 (73.3)
  • a Determined in supernatants by using NADH as a substrate. Release of LDH was measured at different gentamicin treatment times after a 2-h infection period and expressed as units per liter of supernatant (means ± standard errors of the means n = 3 independent experiments in duplicate). ND, not determined.

  • b Calculated as (experimental release − spontaneous LDH release at 1 h)/(total LDH release at 1 h − spontaneous release at 1 h) × 100. Spontaneous LDH release by uninfected cells were 14.9 ± 4.2 and 43.2 ± 5.2 after 1 and 24 h of gentamicin treatment, respectively.