Table 1.

Uptake and ability of E. coli strains isolated from patients with CD to evade killing by J774-A1 cells after 1, 4, 8, 24, and 48 h of gentamicin treatment

MOI and strainUptake of bacteria (104CFU)a after 1 h of treatment% Survival of bacteriab after the indicated treatment period
4 h8 h24 h48 h
MOI = 10
 K-12 C6002.4 ± 0.2 122 ± 5124 ± 11 6 ± 1 0.6 ± 0.1
 LF8212.6 ± 1.1 217 ± 31 280 ± 40 504 ± 631122 ± 297
 LF9 32.5 ± 20.3 132 ± 22201 ± 85 969 ± 261 1743 ± 798
 LF1517.9 ± 6.7 74 ± 7 98 ± 6 624 ± 212700 ± 57
 LF16 18.5 ± 4.8 162 ± 25205 ± 20 3537 ± 418 7421 ± 1246
 LF288.5 ± 2.2 213 ± 92 212 ± 62 563 ± 120291 ± 85
 LF31 8.1 ± 3.6 130 ± 6146 ± 21 850 ± 137 1676 ± 317
 LF4315.1 ± 7.5 258 ± 71 455 ± 124 820 ± 164910 ± 319
 LF46 0.5 ± 0.04 364 ± 160670 ± 221 2782 ± 582 4286 ± 633
 LF507.5 ± 1.0 156 ± 19 346 ± 13 2071 ± 4905506 ± 417
 LF65 12.1 ± 2.1 286 ± 42423 ± 81 1239 ± 31 3267 ± 44
 LF714.8 ± 0.6 259 ± 28 600 ± 213 646 ± 278782 ± 277
 LF100 13.8 ± 2.9 191 ± 16248 ± 46 251 ± 47 265 ± 24
 LF10633.1 ± 1.0 156 ± 24 184 ± 18 365 ± 169225 ± 106
 LF107 2.2 ± 0.8 147 ± 11420 ± 186 572 ± 237 591 ± 212
 LF12010.5 ± 1.2 158 ± 21 167 ± 67 495 ± 1371451 ± 274
MOI = 10
 K-12 C600 6.7 ± 1.8 145 ± 4 ND2.18 ± 1.10 0.8 ± 0.1
S. entericaserovar Chloeraesuis 253.0 ± 61.2 134 ± 29 ND0.04 ± 0.02 ND
S. flexneri M90T 10.4 ± 1.2 57 ± 11 ND0.40 ± 0.30 ND
 LF82 75.0 ± 26.0 237 ± 41268 ± 84 450 ± 59851 ± 264
  • a Numbers of CFU recovered from lysed monolayers; means ± standard errors of the means determined from three to five independent experiments in duplicate.

  • b Mean percentages ± standard errors of the means of intracellular bacteria relative to the number after 1 h of gentamicin treatment, defined as 100%. ND, not determined.