Table 5.

Production of cytokines in stimulated spleen cells from vaccinated or unvaccinated mice challenged with B. abortus2308 and killed 3, 6, and 10 days after challengea

CytokineTreatmentCytokine production (pg/ml)b
3 days6 days10 days
IFN-γNone + challenge807 ± 4651,676 ± 1221,518 ± 249
Vaccination + challenge1,665 ± 409 1,919 ± 2041,404 ± 677
IL-12 p70None + challenge15 ± 137 ± 112 ± 3
Vaccination + challenge22 ± 1200
IL-12 p40None + challenge168 ± 7277 ± 47417 ± 290
Vaccination + challenge184 ± 56252 ± 192 223 ± 133
IL-10None + challenge018 ± 1971 ± 55
Vaccination + challenge35 ± 2935 ± 5291 ± 79
IL-4None + challenge9 ± 532 ± 1610 ± 12
Vaccination + challenge9 ± 531 ± 269 ± 4
  • a BALB/c mice were treated as shown, and cytokine productions in stimulated spleen cells were assessed at 3, 6, and 10 days after challenge. Data are mean values of five animals ± SD.

  • b Groups with different number of asterisks are statistically different compared to unavaccinated controls (P ≤ 0.05) .