Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesReference or source
E. coli
 DH5α endA1 hsdR17(rK mK )supE44 thi-1 recA1 gyrA relA1Δ(lacZYA-argF) U169deoR[f80dlacΔ(lacZ)M15]44
 SM10 thi thr leuB tonA lacY supE recA::RP4-2-Tc::Mu-Km Kmr 46
 CFT073UTI clinical isolate33
 CFT073-TB tonB This study
 CFT073-LH chuA::cam This study
 CFT073-ENT entF::kan This study
 CFT073-IUC iucB::cam This study
 CFT073-ENT/IUC entF::kan iucB::cam This study
 CFT073-IUT iutA::cam This study
Plasmids
 pBluescript SKCloning vector; Cbr Stratagene
 pWKS30Cloning vector; Cbr 53
 pWSc-1pWKS30 containing sacBgene; Cbr Sucs 38
 pHM5Suicide vector; Cbr Sucs 42
 pCVD442Suicide vector; Apr Sucs 9
 pUC-4K kan cassettePharmacia
 pMA9 cam resistance gene20
 pYUK1 tonB in pACYC177; Cmr This study
 pUCHUA::Cm chuA::camin pHM5This study
 pIUCB::Cm iucB::camin pWSc-1This study
 pIUTA::Cm iutA::camin pCVD442This study
 pUENTF::Km entF::kanin pWSc-1This study