Table 2.

Sequences of PCR primers used in this study

Primer Sequence (5′-3′) Incorporated site
Primer 1 (luxA forward)GCA CGA ATT CGT CGA CAG GAG GAC TCT CTA TGA AAT TTG GAA ACEcoRI
SalI
Primer 2 (luxB reverse)CAA CTC GGT ACC TAT TAG GTA TAT TTC ATG TGGKpnI
Primer 3 (luxC forward)CCC CGG TAC CAG GAG GAA GGC AAA TAT GAC TAA AAA AAT TTCKpnI
Primer 4 (luxD reverse)CTC AGG ATC CTT TAA GAC AGA GAA ATT GCBamHI
Primer 5 (luxE forward)CCC GGA TCC TGA GGA GGA AAA CAG GTA TGA CTT CAT ATGBamHI
Primer 6 (luxE reverse)GGG TTA GCT GCA GGA TAT CAA CTA TCA AAC GCPstI
EcoRV
Primer 7 (P3 forward) CAC CGA ATT CCT CAC TGT CAT TAT ACGEcoRI
Primer 8 (P3 reverse)CAT CAA CCC CGG GCC ATC ACA TCT CTG TCA TCT AGSmaI