Table 2.

Sequences of PCR primers used in this study

Primer Sequence (5′-3′) Incorporated site
Primer 1 (luxA forward) GCA CGA ATT CGT CGA CAG GAG GAC TCT CTA TGA AAT TTG GAA AC EcoRI
SalI
Primer 2 (luxB reverse) CAA CTC GGT ACC TAT TAG GTA TAT TTC ATG TGG KpnI
Primer 3 (luxC forward) CCC CGG TAC CAG GAG GAA GGC AAA TAT GAC TAA AAA AAT TTC KpnI
Primer 4 (luxD reverse) CTC AGG ATC CTT TAA GAC AGA GAA ATT GC BamHI
Primer 5 (luxE forward) CCC GGA TCC TGA GGA GGA AAA CAG GTA TGA CTT CAT ATG BamHI
Primer 6 (luxE reverse) GGG TTA GCT GCA GGA TAT CAA CTA TCA AAC GC PstI
EcoRV
Primer 7 (P3 forward) CAC CGA ATT CCT CAC TGT CAT TAT ACG EcoRI
Primer 8 (P3 reverse) CAT CAA CCC CGG GCC ATC ACA TCT CTG TCA TCT AG SmaI