Table 1.

Comparison of cytokine gene expressions in the stomachs of nTx mice with or without H. pylori infection as determined by RT-PCR

CytokineGroupaPositive prevalence (no. of animals positive/ total no. of animals) Relative intensity of mRNAb ± SD
IFN-γnTx 8/8469.00 ± 109.00
nTx+Hp10/10 488.95 ± 96.23
IL-7 nTx 8/8153.45 ± 17.97
nTx+Hp 10/10 109.33 ± 33.26
IL-7R nTx 8/8 404.73 ± 43.70
nTx+Hp10/10 519.42 ± 61.39
IL-4 nTx 1/830.15 ± 32.01
nTx+Hp 8/10 168.80 ± 17.57
TNF-α nTx 6/8 91.34 ± 32.90
nTx+Hp7/7 237.68 ± 39.73
LT-α nTx 2/865.93 ± 36.97
nTx+Hp 9/10181.29 ± 41.61
  • a nTx, thymectomized mice withoutH. pylori infection; nTx+Hp, thymectomized mice withH. pylori infection.

  • b Values are means ± the standard deviations. The densities of the bands were measured by an image autoanalyzing system (Fotodyne, FOTOanalyst, and Archive ECLIPSE) and corrected by using the β-actin density of each sample. Samples were obtained from the mouse stomachs 12 weeks after H. pyloriinfection. For the last three pairs of values (i.e., for IL-4, TNF-α, and LT-α), the values for the infected animals were significantly different (P < 0.05) from the values for the noninfected nTx mouse group as calculated by using the Wilcoxon t test.