Table 1.

C. pneumoniae infection inhibits the appearance of staurosporine- and CD95- and DNP-induced nuclear apoptosis in HeLa cellsa

Exp and treatment% Cells (SD) with apoptotic nuclear morphology
Mock infectedC. pneumoniae infected
Exp 1
 None0.57 (0.18)0.43 (0.38)
 Staurosporine61.23 (7.24)17.27 (5.91)
Exp 2
 None0.9, 0.33.0, 2.0
 Anti-CD95 and DNP40.1, 34.014.5, 14.9
  • a HeLa cells were either mock infected or infected with C. pneumoniae at about 1.5 IFU per cell. For experiment 1, on day 3 of infection, staurosporine (1 μM) was added to one part of the cultures. Four hours later, cells were stained with Hoechst dye and scored for nuclear apoptosis under a fluorescence microscope. Numbers give percentages of apoptotic cells (mean and standard deviation of the mean for three separate wells for each condition; at least 300 nuclei per sample were counted). The data shown are representative of four separate experiments, where apoptosis was induced on either day 2 or day 3 of infection. For experiment 2, on day 2 of infection, anti-CD95 MAb CH11 (100 ng/ml) and DNP (1 mM) were added to duplicate wells. Five hours later, cells were stained with Hoechst dye and nuclear morphology was scored as described above. The numbers give the percentages of apoptotic nuclei for each of the duplicate cultures. This result is representative of two similar experiments.