Table 2.

Reactivity in iELISA of the twelve Omp31-specific MAbs with B. ovis 020, recombinant E. coli(pNV3147), and recombinant E. coli (pNV3123)

MAbODawith:
B. ovis 020 (ns)B. ovis 020 (s)E. coli (pNV3147) (s) (B. ovisOmp31)E. coli (pNV3123) (s) (B. melitensis Omp31)
8F2>3.500>3.500>3.5000.212
9C2>3.500>3.5001.7290.075
11E7>3.500>3.5001.6390.065
12G7>3.500>3.500>3.5000.033
4B20.5620.7190.3510.031
11E31.3421.7500.9390.065
14D50.5660.7530.2700.014
17E81.4951.7661.1160.008
12H90.2060.3360.2140.010
18B70.2400.4360.3640.073
A01/08H06/G020.6400.7890.4510.268
A59/10F09/G10>3.500>3.500>3.500>3.500
  • a OD values lower than 0.100, determined by reactivity of the MAbs with E. coli (pGEM-7Zf) used as negative control, were considered negative. iELISA was performed with either nonsonicated (ns) or sonicated (s) cells. Hybridoma culture supernatants were used diluted 1/5.