Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Relevant features and/or genotypeaSource or reference
Plasmids
 pMK4Gram-positive shuttle vector, Apr, Cmr 43
 pGC4 pMK4 derivative; 8.2-kb plasmid, contains luxAB fusion from Vibrio harveyi expressed from Pxyn, Apr and Cmr C. Rees (unpublished data)
 pSL1190 Superlinker plasmid, contains 64 restriction sites, Apr Pharmacia
 pBluelux luxCDABEfrom P. luminescens inserted intoSmaI site of pBluescript II KS, Apr J. Throup (unpublished data)
 pHG327 Based on pHG165, contains pUC18 multiple cloning site, Apr 42
 pSB2023 luxAB inserted into multiple cloning site of pHG327, Apr This study
 pSB2024 luxCDEinserted downstream of luxAB in pSB2023, Apr This study
 pSB2025 luxABCDE operon excised from pSB2024 and ligated with pSL1190, Apr This study
 pSB2019 Translationally enhanced gfp3 downstream of PxylA, Apr Cmr 35
 pSB2030 luxABCDE excised from pSB2025SalI/PstI and inserted downstream of PxylA::gfp (pSB2019), Apr Cmr This study
 pSB2031 P3 promoter amplified by PCR from S. aureus 8325-4, inserted into EcoRI/SmaI site of pSB2019, Apr Cmr This study
 pSB2035 P3 amplified by PCR from S. aureus 8325-4, exchanged with PxylA from pSB2030, AprCmr This study
E. coli JM109Δ(lac-proAB) recA1 endA1 gyrA9b thi hsdR17 supE44 relA1 F′ (traD36 proAB lacI q lacZ1 ΔM15) 48
S. aureus
 8325-4 Wild-type strain, cured of known prophages and plasmids 29
 RN6390 Prototypical wild type 33
 RN4220 Restriction-deficient derivative of 8325-4 (rK mK +)23
  • a Apr, ampicillin resistant; Cmr, chloramphenicol resistant.