Table 1.

Plasmids

PlasmidAssociated eib geneAmino acidsVectorInsert sourceInsert identification
pCS6379a eibA 1–392pOK12pCS61021,390-bpXmnI-MscI
pDC2240a eibA 254–392pMal-c2XpDC6165526-bpHpaI-MscI
pDC2252 eibA 301–392pMal-c2XpDC2240386-bpPstI-MscI
pDC2273b eibA 254–392pMal-c2XpDC2240526-bpHpaI-MscI
pDC2283b eibA 254–344pMal-c2XpDC2273273-bpHpaI-MluI
pCS7299 eibA 254–298pMal-c2XpDC2240136-bpHpaI-PstI
pCS6431a eibC 1–504pOK12pCS63512,463-bpDraI-XmnI
pCS6365a eibD 1–511pOK12pCS63501,894-bpDraI-BglI
pCS6432a eibE 1–487pOK12pCS63981,891-bpDraI-KasI
pCS7164 eibF 1–459pOK12ECOR-23,167-bp partial Sau3A
pCS7180 eibF 1–459pOK12pCS71642,887-bpMluI-Sau3A
pCS7216 eibF 1–459pOK12pCS71642,049-bpXmnI-Sau3A
pCS7217 eibF 1–459pUC21pCS71642,115-bpNruI-Sau3A
pCS7227 eibF 97–459c pMal-c2XpCS72171,657-bpEcoRI-Sau3A
pCS7269 eibF 97–459pMal-c2XpCS72271,657-bpEcoRI-Sau3Ad
pCS7280 eibF 318–459pMal-c2XpCS72171,331-bpBsgI-Sau3A
pCS7284 eibF 400–459pMal-c2XpCS7269748-bpStuI-Sau3A
pCS7285 eibF 97–399pMal-c2XpCS7269909-bpEcoRI-StuI
pCS7286 eibF 181–280pMal-c2XpCS7164300-bp amplicond
pCS7296 eibF 97–353pMal-c2XpCS7269773-bp amplicond
  • a Reference (16).

  • b Eco O109I-EcoRV deletion inlacIQ eliminated MluI site in vector.

  • c Out-of-frame fusion; all other pMal-c2X fusions are in frame.

  • d See Table 2 for details.